Mechanism of N10 -formyltetrahydrofolate synthetase derived from complexes with intermediates and inhibitors
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چکیده
منابع مشابه
Physical properties of formyltetrahydrofolate synthetase from Clostridium thermoaceticum.
The hydrodynamic parameters of the thermostable formyltetrahydrofolate synthetase from Clostridium thermoaceticum have been measured in the ultracentrifuge at 20”. &J,~ = 9.86 f 0.20 S;vzo = 0.752 f 0.011 cc per g; and molecular weight = 244,000 f 9,000. Calculations based on these parameters give a frictional ratio, f/f0 = 1.27 f 0.11, and a Stokes radius of 53.0 f 4.8 A. The sedimentation con...
متن کاملDistribution of 10-formyltetrahydrofolate synthetase in eubacteria.
The distribution of 10-formyltetrahydrofolate synthetase, which activates formate for use as a one-carbon donor in a variety of biosynthetic reactions, was determined for a variety of eubacteria. Organisms from several genera were found to lack detectable synthetase activity; however, all organisms tested were found to contain 5,10-methylenetetrahydrofolate dehydrogenase activity.
متن کاملEquilibrium and water proton relaxation rate enhancement properties of formyltetrahydrofolate synthetase-manganous ion-substrate complexes.
Binding of Mn(pi)-nucleotide complexes to the enzyme formyltertrahydrofolate synthetase (EC 6.3.4.3) from Clostridium cylindrosporum has been examined in the presence and absence of other substrates by solvent proton relaxation mearurements. MnADP and MnATP form ternary complexes with the enzyme with highly enhanced proton relaxation rates for water. The enhancement parameters, epsilont, for th...
متن کاملSynthesis and Characterization of Metal Complexes with New Unsymmetrical Schiff Base Ligand Derived from 2-Aminoethanethiol
A new unsymmetric thioether diamine N - ( 2 - ( 2 - aminophenylthio) ethyl) benzene - 1 , 2 - diamine was prepared through the nucleophilic substitution of cysteaminium chloride with 1 - chloro - 2 - nitrobenzene and then the nitro groups of prepared compuond converted to the amine by zinc powder and ammonium chloride. Then, unsymmetric Schiff bass ligand 2 - ((( 2 - (( 2 - (( 2 - (( 2 - hydrox...
متن کاملHydrogen ion-induced reversible inactivation and dissociation of formyltetrahydrofolate synthetase.
Formyltetrahydrofolate synthetase undergoes inactivation at pH values below 7. Inactivation is accompanied by both the dissociation of the tetramer into monomers and the exposure of aromatic amino acids to the solvent. The rate of inactivation is a function of pH. This rate and the rate of exposure of tryptophan residues to the aqueous environment follow first order kinetics. After prolonged in...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2011
ISSN: 0961-8368
DOI: 10.1002/pro.2005